Serum Tapeworm ELISA validation (see Tapeworm Blood Test page for more information and for peer reviewed publications)

• Validation is published in Veterinary Clinical Pathology. (Lightbody, K. L. et al. (2016) Vet. Clin. Path., 45: 335–346).

• 85% sensitivity and 78% specificity is obtained for a 1+ tapeworm cut-off.

• 15% of horses were misclassified as negative, but no false negatives were obtained for horses with a pathogenic burden (20+ tapeworms).

 

ELISA storage and shelf life

• ELISA plate storage: -20°C.

• Other kit components: 4°C.

• 2 years shelf life at recommended storage temperatures.

• 4 weeks shelf life once ELISA plate seal is broken.

• Storage inter-variability:  5.5% CV for 25 calibrator:  5.9 - 10.9% CV range for samples (n=6)  (17 test kits over 24 week stability trial).

 

ELISA precision

• Intra-variability: 2.5% CV for 25 calibrator: 1.3 - 5.1% CV range for samples (n=8).

• Inter-variability*: 4.6% CV for 25 calibrator: 2.0 - 13.0% CV range for sample (n=8) (8 ELISA plates over 5 days).

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Reagents and materials provided

• ELISA plate pre-sensitised with tapeworm antigens.

• Vacuum packed ELISA plate in stripwell format allowing three separate uses of the kit if required.    

  • 3 uses = up to 33 samples

  • 2 uses = up to 38 samples

  • 1 use = up to 43 samples

• Calibration reagent supplied – anti-tapeworm IgG

  • Calibration curves must be performed for each ELISA test for quality control and to calculate  serum scores.

• Anti-horse IgG(T):Horseradish Peroxidase (HRP) conjugate.

• 3,3’,5,5’-Tetramethylbenzidine (TMB) Liquid Substrate System.

 

Key Materials and Equipment required but not provided include:

• Microbalance

• Incubator

• Plate reader capable of absorbance readings at 450 nm

• Pipettes including multichannel pipette

• Plate washer (preferable)

• PBS tablets

• Tween 20

• Bovine Serum Albumin (Sigma – A6947)

• Plate sealing films if running >1 test

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Protocol Outline​ 

1. Prepare all reagents, samples and calibrators as detailed in the instructions.

2. Add blocking buffer to each well.

3. Incubate for 1 hour at 25°C.

4. Add samples and calibrators to each well in duplicate.

5. Incubate for 1 hour at 25°C.

6. Add TMB to each well.

7. Incubate for 10 minutes at 25°C.

8. Add stop solution to each well.

9. Read plate at 450nm.

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Number of samples tested by one kit

• three separate uses: 33

• two separate uses: 38

• one use: 43

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Product Safety

MSDS documentation can be downloaded in the link below with password provided upon order

​For trading in the EU: We ensure that all our products comply with GPSR. 
EU Responsible Person (authorities only):
Eucomply OU
Parnu mnt 139b-14
11317 Tallin, Estonia
hello@eucompliancepartner.com
+33757690241
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